well.....after all blog is a personal online journal !!!one is free to write whatever one feels like...
it was a DAY!!!!!!!!!!!! in true sense !!!!!!!
phase 1: 9:00 am
entered d lion's den ( dats my lab) at 9 o clock in the morning and started d most hated job!!!! to clean d laminar flow chamber ( its a mandatory job for any molecular biological experiments to ensure sterile environment for DNA-RNA)in a dreaded place known as "biohazard lab"!!!! then d ordeal started .....the reagents started treachery and started playing hide and seek......compelling me to almost get my head in the -20 degree refrigerator !!! (DNA and reagents are more precious than your brain !!!! after all d university pays for them not for your brain !! u block :P ) however jei ekta bijoyi haashi niye shubharambh korte jabo....emon shomoye d dreadful feline due .two friends (eh !!!!!) appeared from nowhere... " HELLO ELIZA.........we were supposed to work !!!!!" with an expected taken for granted "Sorry" from eliza.......kaaj er ogroni howar oporathe lojjito noto shire !!!!!!
phase 2 : 11:30 am
tarpor experiment er second phase ....amar ordeal er o second phase ......PCR theke tube bar korte jabo RFLP lagabo.....(PCR and RFLP = technical jargon....details if interested wikipedia ) :D abar dui manik jod(i) r punarbhibhav !!!!! " plz!!!! i wanted to use this cycler !!!! why on earth did u put up ur reaction on dis? " (mujhe sapne aane waale the ki she needed dat particular cycler today ) (:))
phase 2 cont:
RFLP lagate jabo........"eliza !!!!!! tu kitne der tak laminar me baithi rahegi ? ( as if laminar is d beach side where i m with my boy friend )maine tujhe msg kiya tha ki i ll be back after some time ? " ( airtel network never helps one even during emergency in d campus beleive it or not..... i dont know why she messeged when she was just 4 rooms away from the lab...india is shining technically after all ) :P :D
phase 3: 5:15 pm
visualization of DNA requires a technique called gel electrophoresis which uses a substance called agarose ( A relatively expensive powder). standard protocol onushare 8 khana sample load kora jaye ei hishebe e gel banate lagey !! r jodi sample kom thake
tahole
du bhage kete rakhte hoye (bachat is a good habit..it helps gals later in life .. :D)
to i required only 4 samples and forgot to SAVE!!!!!!!!! so u know...ur boss is more strict than ur husband !!! (husbands do consider eh !) i had to DEFEND my self justifying why i didn't save "agarose is expensive" (after all university is not able to manage 300 crores unspent cash )
phase 4: 6:30 pm
shamne phd entrance.....cheshta korte khoti nei......tried to concentrate for a change on d pescribed book!!!! for a change :D :P was studying in d porch...lab e firte giye dekhi...bondhoduar.....konthoswor er prayog ta bhodroshomaj er riti niti r khilaaf.....gentlemanly (gentle ladily) bell ke birokto korar dushahosh korlam......
ek bar
.
.
.
.
dui bar
.
.
.
tin bar
.
.
.
choturtho bar...........nah ar para galo na....."kya re mujhe koi kaam nahi hai kya tere liye darwaza khoolne ke alawa " (1. to fir ma janani..dorja ta bondho korte ke bolechhilo? 2. shamnei to boshe chhilam....bolte ki hoyechhilo....ki r kori bhogoban ke bollam...thakur eto kichhu dile mathar pechhon e ek joda chokh dite parle na eki obichar tomar doyamoye? obolar r ki chayi oi extra ek joda chok chhara? )
phase 4 (7 pm)
er pore ki r oi lion's den e thaka jaye????????????
ki r kori....ghorer kotha mone porlo...... home sweet home bole kotha....subah ka bhula shaam ko ghar aaye to bhula nahi kahte!!!!
kintu haaye re pora kopal !!!!!!
janoni kitchen e byasto tadka lagate......haye re jononir bhulo mon............lonka ta tel e diye ei shontan snehe mugdho hoye je chhute elen...lonka ke eka chhere diye....lonka ki r nijer beizzati shojjo korte pare...protihinshar agune jwole uthhlo......
10 35 pm!!!! i m still coughing with a saridon failed constant headache !!!!!
( a line of confession: dis blog has been written in order to divert the stress and sorrow... :) )
